Inverted microscopes require slides to be loaded upside down. Need to find your objective’s working distance? Just check the inscribed specifications or our online objective catalog.įigure 1. Working distances can vary from a little more than 100 µm to several millimeters. In simple terms, the working distance is how close the objective must be to focus on the specimen. When loading the sample, pay special attention to the objective’s working distance. This liquid may corrode or oxidize optical coatings, optical glue, or other parts of the microscope hardware and electronics. If the sample isn’t well sealed, liquid can make contact with your objectives or objective nosepiece. With inverted microscopes, it is important to check that your sample is well sealed. Always load coverslips facing up on an upright microscope and down on an inverted microscope. Next, load the prepared sample in the correct orientation for your microscope. Facial tissues or lab tissues are abrasive and can damage optical surfaces. When cleaning optics, always use lens paper. Another option is an optical cleaner, such as Sparkle Optical Lens Cleaner by A.J. A good cleaning solution is 70% ethanol in distilled water. This causes strong background in blue and green channels, and sometimes in the red channels.Ĭleaning the coverslip and slide before imaging is also important. But in fluorescence imaging, plastic is very autofluorescent. For some applications, plastic slides are acceptable. Make sure to verify your coverslip thickness and use standard glass slides. If the coverslip is too thick or too thin, optical artifacts can occur. Olympus objectives that are designed for coverslips need the right thickness for good image quality. Here are some helpful tips:įirst, select the appropriate coverslip. Once your equipment is powered up, take the time to properly prepare, load, and level your sample for imaging. Launch the software: If you encounter errors during the software launch, we’re here to help! Reach out to your local sales representative or contact us here for technical assistance.Consult your manual for specific instructions. For instance, the CBH on Olympus systems must be powered on before the TPC. ![]()
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